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Using b As New Bitmap(1, 1)
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Using g As Graphics = Graphics.FromImage(b)
g.CopyFromScreen(New Point(300, 300), New Point, New Size(1, 1))
PictureBox1.BackColor = b.GetPixel(0, 0)
End Using
End Using
复制当前屏幕左上角位置（300,300）的一个点，取其颜色
VB可使用Point方法来获取图片指定点的颜色。
Point 方法
按照长整数，返回在 Form 或 PictureBox 上所指定磅的红-绿-蓝 (RGB) 颜色。
语法
object.Point(x, y)
'窗体判色代码:
Private Sub Form1_MouseMove(Button As Integer, Shift As Integer, X As Single, Y As Single)
Text1 = X
Text2 = Y
Text3 = Point(X, Y)
Text4 = (Val(Text3) Mod 65536) Mod 256 'Red
Text5 = (Val(Text3) Mod 65536) \ 256 'Green
Text6 = Val(Text3) \ 65536 'Blue
Shape1.FillColor = RGB(Val(Text4), Val(Text5), Val(Text6))
End Sub
'PictureBox判色代码:
Private Sub Picture1_MouseMove(Button As Integer, Shift As Integer, X As Single, Y As Single)
Text1 = X
Text2 = Y
Text3 = Picture1.Point(X, Y)
Text4 = (Val(Text3) Mod 65536) Mod 256 'Red
Text5 = (Val(Text3) Mod 65536) \ 256 'Green
Text6 = Val(Text3) \ 65536 'Blue
Shape1.FillColor = RGB(Val(Text4), Val(Text5), Val(Text6))
End Sub
我有个笨办法，先用API抓图到内存里，然后再在根据你点鼠标的屏幕工作区坐标，去那图里取色。
-----------------------
'抓图所需的API
Private Declare Function CreateCompatibleDC Lib "GDI32" (ByVal hDC As Integer) As Integer
Private Declare Function CreateCompatibleBitmap Lib "GDI32" (ByVal hDC As Integer, ByVal nWidth As Integer, ByVal nHeight As Integer) As Integer
Private Declare Function SelectObject Lib "GDI32" (ByVal hDC As Integer, ByVal hObject As Integer) As Integer
Private Declare Function BitBlt Lib "GDI32" (ByVal srchDC As Integer, ByVal srcX As Integer, ByVal srcY As Integer, ByVal srcW As Integer, ByVal srcH As Integer, ByVal desthDC As Integer, ByVal destX As Integer, ByVal destY As Integer, ByVal op As Integer) As Integer
Private Declare Function DeleteDC Lib "GDI32" (ByVal hDC As Integer) As Integer
Private Declare Function DeleteObject Lib "GDI32" (ByVal hObj As Integer) As Integer
Declare Function GetDC Lib "user32" Alias "GetDC" (ByVal hwnd As Integer) As Integer
Const SRCCOPY As Integer = HCC0020
'抓图的部分
Dim hDC, hMDC As Integer
Dim hBMP, hBMPOld As Integer
Dim sw, sh As Integer
hDC = GetDC(0)
hMDC = CreateCompatibleDC(hDC)
sw = Screen.PrimaryScreen.Bounds.Width
sh = Screen.PrimaryScreen.Bounds.Height
hBMP = CreateCompatibleBitmap(hDC, sw, sh)
hBMPOld = SelectObject(hMDC, hBMP)
BitBlt(hMDC, 0, 0, sw, sh, hDC, 0, 0, SRCCOPY)
hBMP = SelectObject(hMDC, hBMPOld)
Dim bmp As Bitmap = Image.FromHbitmap(New IntPtr(hBMP))
DeleteDC(hDC)
DeleteDC(hMDC)
DeleteObject(hBMP)
......
'取点的颜色
bmp.GetPixel(e.X, e.Y)
----------------------------
关键就是这些你自己组合吧，你分给的太少了，很麻烦，恕我不帮你改全了。如果要仔细帮你改，请另开高分贴，不要用新马甲来
要使用GetPixel函数来取得像素的颜色值，代码如下：
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private void button1_Click(object sender, EventArgs e)
{
Color color = new Bitmap(pictureBox1.Image).GetPixel(10, 10);
MessageBox.Show(color.ToString());