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我有个笨办法，先用API抓图到内存里，然后再在根据你点鼠标的屏幕工作区坐标，去那图里取色。
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'抓图所需的API
Private Declare Function CreateCompatibleDC Lib "GDI32" (ByVal hDC As Integer) As Integer
Private Declare Function CreateCompatibleBitmap Lib "GDI32" (ByVal hDC As Integer, ByVal nWidth As Integer, ByVal nHeight As Integer) As Integer
Private Declare Function SelectObject Lib "GDI32" (ByVal hDC As Integer, ByVal hObject As Integer) As Integer
Private Declare Function BitBlt Lib "GDI32" (ByVal srchDC As Integer, ByVal srcX As Integer, ByVal srcY As Integer, ByVal srcW As Integer, ByVal srcH As Integer, ByVal desthDC As Integer, ByVal destX As Integer, ByVal destY As Integer, ByVal op As Integer) As Integer
Private Declare Function DeleteDC Lib "GDI32" (ByVal hDC As Integer) As Integer
Private Declare Function DeleteObject Lib "GDI32" (ByVal hObj As Integer) As Integer
Declare Function GetDC Lib "user32" Alias "GetDC" (ByVal hwnd As Integer) As Integer
Const SRCCOPY As Integer = HCC0020
'抓图的部分
Dim hDC, hMDC As Integer
Dim hBMP, hBMPOld As Integer
Dim sw, sh As Integer
hDC = GetDC(0)
hMDC = CreateCompatibleDC(hDC)
sw = Screen.PrimaryScreen.Bounds.Width
sh = Screen.PrimaryScreen.Bounds.Height
hBMP = CreateCompatibleBitmap(hDC, sw, sh)
hBMPOld = SelectObject(hMDC, hBMP)
BitBlt(hMDC, 0, 0, sw, sh, hDC, 0, 0, SRCCOPY)
hBMP = SelectObject(hMDC, hBMPOld)
Dim bmp As Bitmap = Image.FromHbitmap(New IntPtr(hBMP))
DeleteDC(hDC)
DeleteDC(hMDC)
DeleteObject(hBMP)
......
'取点的颜色
bmp.GetPixel(e.X, e.Y)
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关键就是这些你自己组合吧，你分给的太少了，很麻烦，恕我不帮你改全了。如果要仔细帮你改，请另开高分贴，不要用新马甲来
要使用GetPixel函数来取得像素的颜色值，代码如下：
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private void button1_Click(object sender, EventArgs e)
{
Color color = new Bitmap(pictureBox1.Image).GetPixel(10, 10);
MessageBox.Show(color.ToString());
Using b As New Bitmap(1, 1)
Using g As Graphics = Graphics.FromImage(b)
g.CopyFromScreen(New Point(300, 300), New Point, New Size(1, 1))
PictureBox1.BackColor = b.GetPixel(0, 0)
End Using
End Using
复制当前屏幕左上角位置（300,300）的一个点，取其颜色
' 全屏幕取色(anbert原创)
' 在窗体上按住鼠标不放，然后可以在整个屏幕移动
' Label控件(就是标签)上会显示取到的颜色，窗口标题显示颜色值
'
' 新建一个窗体，添加一个Label控件，名字叫Label1
' 然后添加以下代码
'
Option Explicit
'声明API
Private Declare Function WindowFromPoint Lib "user32" (ByVal xPoint As Long, ByVal yPoint As Long) As Long
Private Declare Function GetPixel Lib "gdi32" (ByVal hdc As Long, ByVal X As Long, ByVal Y As Long) As Long
Private Declare Function GetCursorPos Lib "user32" (lpPoint As POINT) As Long
Private Declare Function ScreenToClient Lib "user32" (ByVal hWnd As Long, lpPoint As POINT) As Long
Private Declare Function GetDC Lib "user32" (ByVal hWnd As Long) As Long
'自定义一个类型
Private Type POINT
X As Long
Y As Long
End Type
'取到的颜色保存在这里
Dim theColor As Long
'按下鼠标时指针变成十字型
Private Sub Form_MouseDown(Button As Integer, Shift As Integer, X As Single, Y As Single)
Me.MousePointer = 2
End Sub
'全屏幕取色
Private Sub Form_MouseMove(Button As Integer, Shift As Integer, X As Single, Y As Single)
On Error Resume Next
If Button 1 Then Exit Sub
Dim pos As POINT
GetCursorPos pos
Dim hdc As Long, dc As Long
hdc = WindowFromPoint(pos.X, pos.Y)
ScreenToClient hdc, pos
dc = GetDC(hdc)
theColor = GetPixel(dc, pos.X, pos.Y)
Me.Caption = CStr(theColor)
Debug.Print "Color:" CStr(theColor) vbCrLf
Label1.BackColor = theColor
End Sub
'松开鼠标时指针变成默认
Private Sub Form_MouseUp(Button As Integer, Shift As Integer, X As Single, Y As Single)
Me.MousePointer = 0
End Sub
加入一个TextBox控件,一个Command控件
代码:
Private Declare Function GetPixel Lib "gdi32" (ByVal hDC As Long, ByVal X As Long, ByVal Y As Long) As Long
Private Declare Function GetWindowDC Lib "user32" (ByVal hWnd As Long) As Long
Private Sub Command1_Click()
Dim Color As Long
WindowDC = GetWindowDC(0) '获取屏幕的设备场景
Color = GetPixel(WindowDC, 500, 100) '获指定点的颜色
'分解RGB颜色值
R = (Color Mod 256) '红色
b = (Int(Color \ 65536)) '蓝色
G = ((Color - (b * 65536) - R) \ 256) '绿色
Text1.BackColor = RGB(R, G, b)
End Sub